Ch.3: Antimicrobial Properties of Crustacean Chitosan on Staphylococcus Aureus(ATCC6538) and Candida Albicans (ATCC 10231)

ABSTRACT:- In recent years, there has been a rise in drug resistance in various microbes. This led to the necessity of the discovery of new bioactive compounds with antibacterial and antifungal activity. Thus, an attempt has been made to exWact chitosan from shells of freshwater crabs, Maydel liathel phusa mas oniana, and freshwater prawn, Macrobrachium dayanum. These crustaceans are commonly found in Jharkhand’s ponds, rivers, and paddy fields. These are edible crustaceans, relished by the local people of Jharkhand. An assay of chitosan was done at different concentrations (1.25 mg/ml, 2.5 mg/ml, 3.75 mg/ml, and 5 mg/ml) using the agar well diffusion method against S. aureus (ATCC 6538) and C. albicans (ATCC 10231). The result showed the antibacterial activity against S. aureus was a zone of inhibition formed by M. masoniana chitosan, was 12.0+0.2 mm at 1.25 mg/ml, 13.03+0.05 mm at 2.5 mg/m1,14. 16+0.15 mm at3.75 mg/ml and 15.13+0.15 mm at5 mg/ml. The positive control, Ciprofloxacin (5pg/ disc) showed 25.03+0.05 mm inhibition zone. M. doyoiixm chitosan against S. aureus showed 14.03+0.20 mm at 1.25 mg/ ml, 15.16+0.11 mm at 2.5 mg/ml,16.16+0.15 mm at 3.75 mg/ml and 17.20+0.20 mm at 5 mg/ml inhibition zone. The positive control, Ciprofloxacin (5pg/ disc) showed a 28.03+0.05 mm inhibition zone.